RealHelix™ Superplex qPCR Kit [Probe] 는 단일 반응으로 다양한 표적 증폭 및 검출을 수행하도록 설계되었습니다. 본 제품은 chemically modified hot-start Taq polymerase, uracil-DNA glycosylase (UDG)와 함께 buffer, Mg²⁺, dNTPs 및 안정제(stabilizers)가 혼합된 2배 농도의 premix 제품입니다. 적용된 hot-start Taq polymerase로 인하여 비특이적(non-specific) 증폭을 최소화하고 target DNA만 특이적으로 증폭할 수 있습니다. 키트내에 별도로 포함된 ROX dye는 passive reference dye로 사용될 수 있습니다.

Figure 1. Multiple amplification of five targets in a reaction by RealHelix™ Superplex qPCR Kit [Probe].

Five targets labeled with different fluorescent reporter dyes were analyzed with this Superplex qPCR Premix [Probe]. Reactions were performed using the RealHelix™ Superplex qPCR Kit [Probe] on a real-time PCR instrument, Bio-Rad CFX96.

Figure 2. Twelve-targets multiplex amplification in a reaction by RealHelix™ Superplex qPCR Kit [Probe].

Using the allele-specific FMMA probes (labeled with FAM, HEX, Texas Red, or Cy5), twelve rice SNP genotypes were identified in a single reaction. A mix of rice genomic DNAs (total 10 ng) was used for the FMMA reaction to detect all of the 12 SNPs screened for determination of rice variants. The multiplex reaction was performed with the RealHelix™ Superplex qPCR Kit [Probe] on a real-time PCR instrument, Bio-Rad CFX96.

Storage

• Store below -20 ℃
• ROX dye should be stored in a dark place.

Shelf life

• 12 months